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Sequential protein extracts were prepared from adult male fly heads at different ages (1-day to 4-weeks) and used for Western blot analyses of Ref(2)P and ubiquitinated proteins. The Triton-X Fraction contains the Triton X-100 soluble proteins, while the SDS Fraction identifies the Triton X-100 insoluble/SDS-soluble proteins that have taken on aggregate-like characteristics.
