Table 1. Signal assignments and relative contents of compounds identified in the 1H NMR spectra of different extracts.
label | chemical shift | green cultures* | chlorotic cultures* | compound | |
---|---|---|---|---|---|
AQUEOUS EXTRACT | a | 1.2, 2.5, 4.15 | 5 | 2 | β-hydroxy butyric acid |
b | 3.25 | 2 | 0 | glycinebetaine | |
c | 1.35 | 0.7 | 0.1 | lactic acid | |
d | 1.9 | 2 | 3 | acetic acid | |
e | 3.5–4.0, 5.4 | 12 | 0.2 | sugars | |
METHANOLIC EXTRACT | d | 2.9 | 1.3 | 5 | acetic acid |
e | 3.5–4.0 | 4 | 0.5 | sugars | |
h | 8.55 | 0.1 | 1.6 | formic acid | |
i | 5.96, 6.16, 8.03, 8.45, 9.35, 9.6 | 0.8 | 0 | chlorophyll a | |
j | 5.3, 5.4 | 9 | 3 | unsaturated fatty acids | |
k | 0.9, 1.3 | 16 | 17 | saturated fatty acids | |
CHLOROFORMIC EXTRACT | k | 0.9, 1.3 | 1.5 | 1.7 | saturated fatty acids |
l | 1.28, 2.55, 5.26 | traces | 0.2 | poly-β-hydroxy butyric acid |
* Intensities were measured relative to the internal standards, Trimethylsilyl Propionic-2,2,3,3,-d4 acid (TMSP) and Tetramethylsilane (TMS).