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. 2015 Jul 16;10(7):e0133165. doi: 10.1371/journal.pone.0133165

Fig 5. IL-8 production was increased after activation of EGFR and ERK1/2 by ATP.

Fig 5

2B4 and 1E8 cells were pretreated with AG1478, U0126 or DMSO for 30 min, respectively. Then cells were incubated with or without 100 μM ATP for 12 h. (A) and (B) mRNA level of IL-8 was detected by real-time PCR. (C) and (D) Protein level of IL-8 in cell supernatant was examined by ELISA assay. Results were demonstrated by histograms to quantify the expression levels. Data were presented as means ± SD (vertical bars). Three independent experiments were performed. *p<0.05.