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Mannan-attachment to nanogels surface was accomplished by amine-carboxylic acid coupling. (A) Molecular structures of mannan and intermediates products, as well as mannan-attached to nanogels surface. (B) In vitro ligand agglutination of unmodified and MN-modified nanogels with Con-A. Optical density was measured at 550 nm. Values represent mean ± SD (n = 3).
