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. 2015 Jul 16;10(7):e0132783. doi: 10.1371/journal.pone.0132783

Fig 5. The effect of primer choice on the observed phylogenetic composition.

Fig 5

(A) Comparison of the phylogenetic composition at phylum level using three different sets of primers (V1-3, V3-4, V4), PCR-free metagenomics (MG), stranded metatranscriptomics (MT), and the theoretical primer-set coverage with 1 mismatch allowed. The data is visualized as a table with the underlying colors visualizing the changes. (B) Comparison of alpha-diversity between the different sets of primers using diversity indices and rank abundance curves.