Fig 4. SIVMAC, HIV-2, and SIVSM transduction of human T cells is less efficient than transduction by HIV-1.
(A) Transduction efficiency of VSV G-pseudotyped two-part vectors for HIV-1NL4-3GFP (white squares), HIV-2RODGFP (grey triangles), or SIVMAC239GFP (black circles) on Jurkat T cells. (B) Chimeric vectors were generated in which gag-pol of SIVMAC239GFP (white squares) was replaced with gag-pol from SIVSME543 (grey triangles) or SIVSM041 (black circles). In each case (A and B), VSV G-pseudotyped vectors were generated by plasmid transfection of 293T cells. Vector stocks were normalized by titer on CRFK cells, and then used to challenge Jurkat T cells. 48 hrs post-challenge, the percentage of GFP-expressing cells was determined by FACS. Data is plotted as percent GFP+ (infected) cells (Y axis) versus CRFK infectious units (IU) x 1000 (X axis).