Fig 8. Knockdown of TRIM5 or of cyclophilin A has no effect on SIVMAC transduction of Jurkat CD4+ T cells.
Jurkat T cells (A) or primary human CD4+ T cells (B) were transduced with lentiviral vectors bearing a puromycin resistance cassette and miR30-based knockdown cassettes targeting either luciferase (black squares), CypA (gray diamonds), or TRIM5 (white triangles). Puromycin-resistant pools of transduced cells were challenged with VSV G-pseudotyped N-MLVGFP, B-MLVGFP, HIV-1NL-GFP, SIVmac-GFP, or EIAVGFP, as indicated. The percentage of GFP+ (infected) cells at 48 hrs is reported. HIV-1NL-GFP and SIVmac-GFP vectors were two-part vectors, with GFP in place of nef. N-MLVGFP, B-MLVGFP, and EIAVGFP were three-part vectors.