Fig 9. The block to SIVMAC infection of Jurkat T cells occurs after formation of 2-LTR circles.
CRFK and Jurkat (A), or Hela and Jurkat (B), or PBMCs (C) were infected with VSV G-pseudotyped HIV-1NL4-3-GFP, or with isogenic vector bearing the SIVMAC239 CA residues 1 to 202. 24 hrs post-infection, DNA was collected from the cells and subjected to qPCR using primers specific for full-length linear viral cDNA, 2-LTR circles, or proviral DNA, as indicated. Shown is the abundance of signal from vector bearing the SIVMAC239 CA1-202, relative to the amount of signal from HIV-1NL4-3-GFP. In each case, infection was performed in the presence of an RT inhibitor to control for background levels of signal.