Figure 4.
Slc22a3 expression. (A) RT-qPCR analyses of 5-HT genes with RNA obtained from unsorted hindbrain dissections of E12.5 Pet-1+/− or Pet-1−/− embryos. (B) Comparative in situ hybridization of Slc22a3 probe in +/+ and Pet-1−/− mice. (C) 5-HT immunohistochemistry and Tph2 in situ hybridization in +/+ and Pet-1−/− adult mice. Scale, 200μm. Genes were analyze by a Student’s t-test. p-value: *p<0.05, **p<0.01, ***p<0.001.