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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Mol Cancer Res. 2015 May 1;13(7):1083–1094. doi: 10.1158/1541-7786.MCR-14-0685-T

Fig. 5. TG2 mutant impact on migration and proliferation in SCC13-TG2-shRNA2 cells.

Fig. 5

A SCC13-Control-shRNA cells were electroporated with 3 μg of empty vector (EV) and SCC13-TG2-shRNA2 cells were electroporated with plasmid encoding TG2-wt, TG2 mutants (C277S, R580A, Y526F or W241A) or empty vector. After electroporation, the cells were seeded at high density in 6-well cluster conventional attachment plates in 2.5 ml of spheroid media. Uniform wounds were created using a pipette and wound width was monitored at 0, 10 and 20 h. An immunoblot confirming mutant expression is shown in Fig. 5B. B Wound images at 20 h. C TG2 knockdown does not alter SCC-13 cell proliferation. SCC13-Control-shRNA and SCC13-TG2-shRNA cells were plated at equal density in attachment plates in spheroid medium and growth was monitored over a period of three days. Values are mean ± SEM, n = 3, p < 0.05.