(A, lower panel) A representative immunoblot to analyze CD73 protein expression in rat CSM after 8 hours incubation with increasing concentrations of sialorphin. (A, upper panel) Densitometry analysis of ratio of CD73 to GAPDH protein expression with increasing sialorphin concentrations. Densitometric analysis was performed from three separate experiments to determine expression of CD73 and GAPDH. Expression of CD73 was normalized to the housekeeping gene GAPDH. The mean fold-change (± Std. Dev.) in expression of CD73 caused by increasing sialorphin concentration relative to untreated controls are shown. *=P<0.05 significantly increased fold-change in protein expression compared to untreated control. (B, lower panel) A representative immunoblot to analyze time dependent protein expression ofCd73 in rat corporal cells with 400 nM sialorphin. (B, upper panel) Densitometric analysis was performed from three separate experiments to determine expression of CD73 and GAPDH. Expression of CD73 was normalized to the housekeeping gene GAPDH. The mean fold-change (± Std. Dev.) in expression of CD73 at different time points, compared to protein expression at baseline (T=0), are shown. *=P<0.05 significantly increased fold-change in protein expression compared to baseline.