Table 1.
Forward and Reverse Primers for Porcine Gene Quantification by Quantitative Real-Time Polymerase Chain Reaction.
| Gene | Primer | Accession Number |
|---|---|---|
| ACOX | 5’TCCAAACGACTGGAGATCA 5’CACAGTAGGGCTATTGAGAA |
JQ087330.1 |
| BSEP | 5’ TTTCATTCAGCGCCTGACCA 5’ ACTCCAATGAGAGGGCTGAC |
XM_003133457 |
| CPT1A | 5’ GGGACAAAAGGCCCTTGTTT 5’ CCAGCACATCTGCACTCAAA |
NM_001129805 |
| CYP2E1 | 5’ CTCCAACCTGCCACATGAAGC 5’ GGAATTACCACTGTGCCCTTG |
NM_214421.1 |
| CYP3A29 | 5’GTGGAGTGTTACATACGGGC 5’AGGTGATACTAGGTGGGGGT |
NM_214423 |
| CYP7A1 | 5’GAAAGAGAGACCACATCTCGG 5’GAATGGTGTTGGCTTGCGAT |
NM_001005352 |
| FGF19 | 5’AAGATGCAAGGGCAGACTCA 5’AGATGGTGTTTCTTGGACCAGT |
ENSSSCG00000012871 |
| FXR | 5’TTTGTGTCGTTTGCGGAGAG 5’GTTGCCCCCATTTTTACACTTG |
XM_003481738 |
| MRP2 | 5’ GAACAGGTTTGCTGGCGATATT 5’ ACCCTGGATGACACCCTCCCC |
DQ530510 |
| OSTα | 5’ TGTACAAGAACACTCGCTGC 5’ GAACACACACACTATCGTGGG |
NM_001244266XM_003132614 |
| SHP | 5’ GCCTACCTGAAAGGGACCAT 5’ CAACGGGTGTCAAGCCTTTA |
DQ002896 |
ACOX, acyl-CoA oxidase; BSEP, bile salt export pump; CPT1A, carnitine palmitoyltransferase 1A; CYP2E1, cytochrome P450 2E1; CYP3A29, cytochrome P450 3A29; CYP7A1, cholesterol 7-hydroxylase; FXR, farnesoid X receptor; MRP2, multidrug resistance-associated protein 2; OST, organic solute transporter; SHP, small heterodimer partner.