Figure 3.

GO-203 increases hydrogen peroxide (H₂O₂) levels in CTCL cells, which is abrogated by addition of N acetyl cysteine (NAC). (A-D) HuT-78 and H9 cells were left untreated and treated with 5 μM GO-203 or CP-2 each day for 3 days. (C and D) The GO-203-treated cells were also incubated in the presence of 5 mM NAC for the last 2 days. Cells were incubated with c-H₂DCFDA for 30 minutes. Fluorescence of oxidized DCF was measured by flow cytometry (left). The results are expressed as the relative hydrogen peroxide level (mean ± standard deviation [SD] of 3 determinations) compared with that obtained for control cells (right).