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. 2015 May 14;309(2):G78–G86. doi: 10.1152/ajpgi.00084.2015

Fig. 4.

Fig. 4.

Gastrin stimulates proMMP-1 expression by AGS-GR cells revealed by Western blot. A: Western blots of HGT1-GR, RMG1-GR, and AGS-GR cells reveal stimulation of proMMP-1 by hG17ns (10 nM) in media (top) and in cell extracts (middle) while there is no difference in cellular GAPDH (bottom). B: concentration-dependent increases in proMMP-1 (52 kDa) in response to hG17ns (1–10 nM). C: stimulation of proMMP-1 by hG17ns (10 nM) is inhibited by the CCK-2 receptor antagonist L740,093 (0.2 μg/ml), while responses to PMA (100 nM) are not; the changes in proMMP-1 can be seen both in media and cell extracts but there is no change in GAPDH abundance in cell extracts. D: hG17ns and PMA increase proMMP-1 abundance in AGS-GR media, but only PMA also stimulates AGS cells which lack the CCK-2 receptor. E: the effects of both gastrin and PMA on proMMP-1 in media are inhibited by a PKC inhibitor, Ro320432 (2.0 μM), and an inhibitor of activation of p42/44 MAP kinase, U0126 (10 μM).