Fig. 5.

Level of Tnf-α and Ifnγ expression in serum and intestinal mucosa of Salmonella-infected mice and sex-matched littermate controls. Fold changes in the serum level of Tnf-α (A) and Ifnγ (B) were measured by ELISA. C: quantitative PCR was performed to determine Tnf-α mRNA expression using total RNA from jejunum (i) and colon (ii). D: quantitative PCR was performed to detect Ifnγ mRNA expression using cDNA prepared from total RNA from jejunum (i) and colon (ii). Values are means ± SE of at least 3–4 separate sets of mice. *P < 0.01.