Fig. 7.

Role of NF-κB in the inhibitory effect of proinflammatory cytokine on biotin uptake by NCM460 cells: translocation of p65 and degradation of IκB. A: Western blot analysis using nuclear (i) and cytosolic (ii) fractions of cytokine-treated NCM460, showing the relative amounts of NF-κB and IκB, respectively. B: effect of the NF-κB inhibitor, MG132, on the proinflammatory cytokine-induced inhibition on SLC5A6-P1 promoter activity (i) and carrier-mediated biotin uptake (ii). Values are means ± SE of at least 3 separate sets of experiments. *P < 0.05, **P < 0.01.