Fig. 7.

SR-nitrite therapy induces eNOS (Ser¹¹⁷⁷) active site phosphorylation and increases NO levels in the heart in obese OS after CLI. A: representative immunoblots of cardiac protein expression of phosphorylated eNOS (Ser¹¹⁷⁷), eNOS (Thr⁴⁹⁵), and total eNOS. B–D: densitometry analysis of total eNOS (B), phosphorylated eNOS (Ser¹¹⁷⁷; C), and phosphorylated eNOS (Thr⁴⁹⁵; D). E: cardiac nitrite levels in CLI + placebo- and CLI + SR-nitrite-treated groups. Values are means ± SE; numbers inside bars are numbers of animals per group.