FIGURE 2.

Workflow of our degradomics approach and criteria for candidate selection. Two groups of cell conditioned media are prepared: one treated with active KLK7 (KLK7 digestion) and one without KLK7 treatment (Control). The cell supernatants are then trypsinized for MS preparation. The processed cell supernatant can, therefore, contain peptides with KLK7/KLK7, KLK7/trypsin, trypsin/KLK7, and trypsin/trypsin cleavage sites. Selection criteria were applied to the list of proteins identified by Mascot and included: unique peptides in KLK7 treatment samples and peptides which have at least one KLK7 cleavage site and proteins with extracellular or cell surface protein localization. For more details see “Results.”