TABLE 1.
Candidate kallikrein 7 substrates based on the screening results from melanoma and HaCaT cell lines
Specific peptide sequence is denoted as the peptide sequence detected by shotgun scanning including the amino acid before and after this sequence (separated by −) to indicate the proteolytic site.
| Protein ID | Specific peptide sequence | Found in cell lines |
|---|---|---|
| MDK | Y-NAQCQETIR-V | WM9,WM902,WM35a |
| CYR61 | R-AQSEGRPCEY-N, R-ICEVRPCGQPVY-S | WM9 |
| TNC | Y-TVTLHGEVR-G | WM9, WM902,WM35 |
| F-TTIGLLYPFPK-D | WM9a | |
| Y-SLADLSPSTHYTAK-I | WM902,WM35 | |
| Y- LSGLAPSIR-T | WM35 | |
| Y-TGEKVPEITR-A | WM35 | |
| R-TAHISGLPPSTDFIVY-L | WM9 | |
| CTGF | Y-RLEDTFGPDPTMIR-A, K-TCACHYNCPGDNDIFESLY-Y | WM9 |
| Isoform 1 of extracellular sulfatase Sulf-2 (SULF2) | Y-HVGLGDAAQPR-N | WM902, WM35 |
| Fructose-bisphosphate aldolase A (ALDOA) | K-CPLLKPWALTF-S, F-LSGGQSEEEASINLNAINK-C | WM9, WM35a |
| WM902 | ||
| Isoform 1 of annexin A2 (ANXA2) | Y-KTDLEKDIISDTSGDFRK-L, | WM9, WM902 |
| Y-TNFDAERDALNIETAIK-T | WM9a | |
| Isoform 1 of complement C1q tumor necrosis factor-related protein 3 (C1QTNF3) | F-AGFLLFETK | WM9, WM35 |
| Spondin-2 (SPON2) | F-SAPAVPSGTGQTSAELEVQR-R | WM902 |
| Complement C4-A (C4A) | F-LSCCQFAESLR-K | WM9 |
| IGFBP3 | Y-KVDYESQSTDTQNF-S | WM902a |
a Peptides also identified in KLK7-treated normal skin HaCaT secretome.