TABLE 2.
Selected reaction monitoring assay validation of specific peptides in cell culture models
Total of five biological samples per condition (control or KLK7 treated samples) were tested in this study.
| Protein ID | Peptides type | Peptides sequence | WM9 |
|
|---|---|---|---|---|
| Control | KLK7-treated | |||
| TNC | Tryptic peptide | ITAQGQYELR | 5/5a | 5/5 |
| KLK7 peptide | TVTLHGEVR | 0/5 | 5/5 | |
| MDK | Tryptic peptide | YNAQCQETIR | 5/5 | 5/5 |
| KLK7 peptide | NAQCAETIR | 0/5 | 5/5 | |
| CYR61 | Tryptic peptide | LPVFGMEPR | 2/5 | 5/5 |
| KLK7 peptide | ICEVRPCGQPVY | 0/5 | 5/5 | |
| CTGF | Tryptic peptide | LPSPDCPFPR | 5/5 | 5/5 |
| KLK7 peptide | RLEDTFGPDPTMIR | 0/5 | 0/5 | |
| IGFBP3 | Tryptic peptide | YGQPLPGYTTK | 5/5 | 5/5 |
| KLK7 peptide | KVDYESQSTDTQNF | 0/5 | 0/5 | |
a x/y = number of times peptide was detected/total number of independent biological samples.