FIGURE 5.

SFN-induced Nrf2 activation inhibited NF-κB signaling pathway in normal BL10 mice. 4-week-old male C57/Black 10 mice were separately treated with SFN (2 mg/kg of body weight by gavage), and 4-week-old C57/Black 10 mice were treated with corn oil in the same dose for 4 weeks. Then mice were sacrificed, and related muscles were collected. A, Western blot of Nrf2, NF-κB(p65), p-IKK-β/IKK-β, p-IKK-α/IKK-α, and p-IκB-α/IκB-α in TA muscles. B, statistical analysis of Western blot of Nrf2, NF-κB(p65), p-IKK-α, p-IKK-β, and IκB-α protein. Data are the means ± S.E. (error bars) of three independent experiments. *, p < 0.05 versus BL10 mice. n = 6 for each group. Statistical analysis was conducted using Student's t test.