TABLE 2.
List of oligonucleotides used in this study
The primers were designed using the Primer 3 program.
| Gene names | Sequences (from 5′ to 3′) |
|---|---|
| gadA-up | TTACCAGGTTGCCGCTTATC |
| gadA-down | ACGCAGACGTTCAGAGAGGT |
| gadB-up | TTACCAGGTTGCCGCTTATC |
| gadB-down | ACGCAGACGTTCAGAGAGGT |
| gadC-up | TCGAAGACCTTCTTCCCTGA |
| gadC-down | CATTACCCGGAATGACCATC |
| gadE-up | TGCCCCATAAGAATTCACAA |
| gadE-down | GTGACGATGTCGCTCATACG |
| hdeD-up | CCGGTATTATCCGGTTTCCT |
| hdeD-down | CTTTCATTGAACGCTGACGA |
| slp-up | GCGAAGCCTGATATTGAAGC |
| slp-down | GGATGCCCTGCATATTCACT |
| gapA-up | AGGTCTGATGACCACCGTTC |
| gapA-down | GGAACGCCATACCAGTCAGT |
| rpoS-up | CCTGCACAAAATTCCACCGTTGC |
| rpoS-down | GATGGGCATCGGACCTTTTATT |
| gadE-P1-up (−195)a | AGGAATCTTACTTAGGATCAATAT |
| gadE-P1-down (+49)a | GCCAAAAGCCCTGTAAAAGAAAAGAATC |
| gadE-P2-up (−360)a | TTGCCAGCTTAAGTCGAAACAAGG |
| gadE-P2down (−190)a | ATTCCTGGTTGTTATCAGCTTGTA |
| gadE-P3-up (−532)a | GCGTTGATGCTATGGGCGGTTAAAT |
| gadE-P3-down (−310)a | ATGTAATCCGATTTAAATATCGAG |
a For the primers of gadE promoters (P1, P2, and P3), the positions are denoted based on +1 as the starting position and designed according to Sayed and Foster (32) and obtained from Sigma Life Sciences.