FIGURE 2.

Changes in the association of specific mRNAs with polyribosomes during mild hyperosmotic stress. A, MEFs were treated with 500 mosmol medium alone or in the presence of Sal (15 μm) for the indicated times, and the distribution of mRNAs in polysomes was analyzed using qRT-PCR. mRNA in each fraction is expressed as the percentage of total mRNA. The dashed lines indicate that fractions 6–12 contain polyribosomes. B, quantification of three independent experiments from A. Error bars represent mean ± S.E.