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Effect of DJ-1 on activation of c-Raf in PMA-treated cells. A, DJ-1+/+ and DJ-1−/− cells were cultured in a medium with 0.1% calf serum for 6 h and treated with 50 ng/ml EGF for 1, 5, and 15 min. Expression levels of proteins were then analyzed by Western blotting as described in the legend for Fig. 4A. B, intensities of bands shown in A were quantified. n is 4.
