FIGURE 7.
Tid1 is a downstream molecule of the IκB kinase complex. A, HT1080 cells stably expressing NS shRNA or Tid1 shRNA were transfected with vector, Tax1 (HTLV-1 Tax), Tax2 (HTLV-2 Tax), or IKKβKA, Total protein lysates were analyzed using antibodies indicated in the figure. B, in HT1080 cells stably expressing NS shRNA or Tid1 shRNA, GFP-LC3 was co-transfected with vector, Tax1, Tax2, or IKKβKA, The cytoplasmic GFP-LC3 puncta was visualized with fluorescence imaging. C, statistical analysis of the experiment in B. D, Tax-expressing HTLV-1-tranformed T cell lines SLB-1 and MT-4 were transduced with lentivirus expressing NS shRNA or Tid1shRNA; total protein lysates were examined using immunoblot with antibodies indicated in the figure. E, in NS shRNA or Tid1shRNA-expressing HEK293 cells, FLAG-Beclin1 was co-transfected with GST or GST-tagged IKKα, IKKβ, and Tax1. GST pulldown assay was performed to determine the interaction of IκB kinase and Beclin1.