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Kinetic analyses of NHE1 mutants in re-entrant loop EL5. NHE1ΔC and mutants Y370C, E395C, G403C, and S405C were stably expressed in AP-1 cells and assayed for their affinities for Na+ (A–D) and H+ (E–H) according to protocols described under ”Experimental Procedures.“ Values represent the mean ± S.E. of 3–9 experiments, each performed in quadruplicate. Error bars smaller than the symbol are absent.
