FIGURE 4.

Effect of increasing Pb on long bone length, bone turnover markers, and osteoblast precursors in sclerostin-deficient mice. A, gross measurements of total femur length were taken and are presented for each treatment group. B, MSC populations from Pb-treated 3-month-old WT and SOST-KO mice were isolated and sorted for their positive surface expression of Sca-1. The percentage and total number of Sca-1⁺ cells were quantified. C–F, serum bone formation marker P1NP (C), resorption markers CTX-1 (D) and TRAP5b (E), and corticosterone (F) were measured using standard ELISA methods. Data represent mean ± S.E. of 5 mice/group. *, p < 0.05, **, p < 0.005 versus 0 Pb WT, #, p < 0.05, ##, p < 0.005 versus 0 Pb KO.