Figure 4.

Structural models of the TMD and ELs of WT FSHR, chimeric EL2M FSHR, and the point mutants L⁵⁰¹F and I⁵⁰⁵V (31). The acquired interactions in chimeric EL2M FSHR and point mutants L⁵⁰¹F and I⁵⁰⁵V with the adjacent residues in EL1 and EL3, which are absent in WT FSHR is depicted. EL2 region in all the models is shown in yellow. The residues involved in interaction in all the four models are circled in cyan (Y⁴¹⁵, L⁵⁰¹/F⁵⁰¹, and M⁵⁰³). Residues showing similar interaction in chimeric EL2M and L⁵⁰¹F mutant are shown in white square boxes (I⁴¹⁷ and I⁵⁰⁵/V⁵⁰⁵). Residues showing similar interaction in chimeric EL2M and I⁵⁰⁵V are shown in green square boxes (Y⁴⁹⁴ and V⁵⁸²). Hydrogen bonds and hydrophobic interactions are depicted in pink and green lines. The models were built using Discovery Studio 3.5 and minimized using Schrodinger 2013 OPLS 2005 force field with default parameters, as described in Banerjee et al. (31).