FIGURE 6.

In-gel activity of Mn(II) oxidation enzymes. (A) Gel was stained with Coomassie. Lane m-1, Precision Plus Protein Dual Xtra Standards; Lane a, whole cell protein (pH 7.2, exponential phase); Lane b, whole cell protein (pH 5.5, exponential phase); Lane c, whole cell protein (pH 7.2, stationary phase); Lane d, whole cell protein (pH 5.5, stationary phase). Triangles 2 and 3 indicated the putative Mn(II) oxidizing enzymes with molecular masses of 140 and 120 KDa, respectively. (B) Gel was stained with 200 μM MnSO₄. The color of the gel was modified to blue in Adobe Photoshop CC to enhance the clarity of the bands. Lane m-2 and m-3, Precision Plus Protein Dual Xtra Standards; Lane e, whole cell protein (pH 7.2, stationary phase); Lane f, whole cell protein (pH 5.5, stationary phase); Lane g, whole cell protein (pH 7.2, exponential phase); Lane h, whole cell protein (pH 5.5, exponential phase). Triangle 1 indicated the putative Mn(II) oxidizing enzyme with a molecular mass between 120 and 140 KDa.