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. 2015 Jul 17;9:272. doi: 10.3389/fncel.2015.00272

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Copyright © 2015 Baltz and Voigt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Double-pulse experiments. (A) (Left) Shows an example of the average responses before (black) and after (red) the subtraction of the average response to single pulses for Δt = 60 ms. The dotted line indicates the time window of the first wave of activity post stimulus (200 ms) which was considered for the analysis. (Right) Similar graph to the one on the left except for Δt = 800 ms. (B) The graph shows the spikes post stimulus the T-pulse, after subtraction of spikes that were evoked by single pulses for one culture without (left) and with (right) chronically blocked GABA_ARs. (C) The summary graph shows the T-pulse responses. The graph is normalized to the average responses of cultures with intact GABA_Aergic synaptic transmission at Δt = 15000 ms (n = 7 control cultures; n = 6 cultures with chronically blocked GABA_Aergic synaptic transmission; 21–26 DIV; asterisks indicate significant differences between control and blocked cultures; *p < 0.05).