FIG 6.

The length of S phase varies according to the percent DNA content in untreated and treated promastigotes of L. donovani. By using the dual-parameter display method, a gate was drawn around the nonclumped cells (R1) (A), and R1-gated cells were divided into three subgates, R2, R3, and R4 (B). (C) We further applied markers (M1, M2, and M3) to subgates R2, R3, and R4 to denote G₂, G₃, and G₄, depicting pre-S phase, S phase, and post-S phase, respectively. (D) Statistical analysis of subgated cells of L. donovani. (E) S phase halt (in terms of percent DNA content) was prominent in cells treated with 25 μg/ml of synthesized Ag NPs, compared to untreated cells. In the “Marker” column, “c” and “e” indicate untreated parasites and parasites treated with 25 μg/ml of synthesized Ag NPs, respectively.