FIG 2.

Tumor colonization of biofilm-defective P. aeruginosa mutants. (a) Wild-type P. aeruginosa PA14 and biofilm-defective mutants (PA14 pqsA::Tn and pelA::Tn) colonizing different tissues of mice. This experiment includes ≥5 mice per group and was performed at least two times. (b and c) Visualization of P. aeruginosa PA14 pqsA::Tn (b) and P. aeruginosa PA14 pelA::Tn (c) by FISH and TEM. Sections of tumor tissue samples were hybridized with a species-specific probe for P. aeruginosa (green) for FISH analysis. In TEM images, arrowheads indicate individual bacterial cells. No aggregate formation of bacteria and no visible matrix material were observed.