TABLE 3.
HIV-1 isolate | Virus subtype | Cell type | Tropisma | EC50 (μM)b | SI50c | EC90 (μM)b | SI90d |
---|---|---|---|---|---|---|---|
XJDC6291 | CRF07_BC | TZM-bl | CCR5 | 0.006 ± 0.001 | 12,735 | 0.025 ± 0.001 | 14,416 |
XJDC6291 | CRF07_BC | PBMCs | CCR5 | 0.003 ± 0.001 | 45,933 | 0.103 ± 0.010 | 2,890 |
020100968 | B′ | TZM-bl | CCR5 | 0.004 ± 0.002 | 18,021 | 0.016 ± 0.003 | 22,329 |
020100968 | B′ | PBMCs | CCR5 | 0.002 ± 0.001 | 68,900 | 0.209 ± 0.014 | 1,424 |
020100311 | B′ | TZM-bl | CCR5/CXCR4 | 0.001 ± 0.001 | 76,410 | 0.007 ± 0.001 | 51,484 |
020100311 | B′ | PBMCs | CCR5/CXCR4 | 0.002 ± 0.001 | 68,900 | 0.119 ± 0.012 | 2,501 |
CYM015 | CRF01_AE | TZM-bl | CCR5 | 0.001 ± 0.001 | 76,410 | 0.053 ± 0.009 | 6,800 |
CYM015 | CRF01_AE | PBMCs | CCR5 | 0.006 ± 0.001 | 22,967 | 0.209 ± 0.014 | 1,424 |
CYM033 | CRF01_AE | TZM-bl | CCR5 | 0.002 ± 0.001 | 39,386 | 0.014 ± 0.003 | 24,958 |
CYM033 | CRF01_AE | PBMCs | CCR5 | 0.005 ± 0.001 | 27,560 | 0.302 ± 0.023 | 985 |
HIV-1 isolate uses CXCR4 (CXC chemokine receptor 4), CCR5 (chemokine receptor 5), or both for cell infection.
EC50s and EC90s were determined on the basis of the data from virus inhibition assays with fresh human PBMCs or TZM-bl cells. The experiment was performed in triplicate, and the data presented are mean values ± standard deviations from at least two independent experiments.
The SI is the ratio of the CC50s for PBMCs, MT-4 cells, and TZM-bl cells to the EC50s for inhibition of clinical HIV-1 isolates of different subtypes.
The SI is the ratio of the CC90s for PBMCs and TZM-bl cells to the EC90s for inhibition of clinical HIV-1 isolates of different subtypes.