TABLE 5.
Sensitivities of molecular clones with RT mutations to WPR-6 and other anti-HIV agents
| WPR-6-selected mutation | EC50 (μM)c (fold differenced) |
||
|---|---|---|---|
| WPR-6 | NVP | EFV | |
| None (WT)a | 0.001 ± 0.0002 | 0.041 ± 0.001 | 0.0008 ± 0.00002 |
| Y188L | 0.009 ± 0.0004 (9) | 10 (>200) | 0.008 ± 0.0002 (10) |
| None (WT)b | 0.001 ± 0.0002 | 0.074 ± 0.015 | 0.001 ± 0.001 |
| V106I | 0.014 ± 0.003 (14) | 0.120 ± 0.009 (1.6) | 0.002 ± 0.001 (2) |
a
A WT HIV-1 strain was used as the control for the Y188L mutant molecular clone.
b
A WT HIV-1 strain was used as a control for the V106I mutant molecular clone.
c
EC50s were determined by virus inhibition assay using TZM-bl cells. The samples were tested in triplicate, and the data presented are mean values ± standard deviations from at least two independent experiments.
d
Fold change was determined by calculating the ratio of EC50s for mutant and WT viruses.