TABLE 2.
Mutations in the embCAB locus that are markers of phylogenetic lineages or potentially associated with ETB resistance in 60 ETB-S and 71 ETB-R isolates of the MTBC
| Mutation in the embCAB locus | Mutation |
Phylogenetic lineage(s) | |
|---|---|---|---|
| Amino acid | Nucleotidea | ||
| Markers of phylogenetic lineages | EmbC R738Q | CAS | |
| EmbC V981L | Haarlem; all H1; all X; all ambiguous T2T3 and T2X1; some T1, T2, and T5; and one each of Manu2, Ghana, and T3 variant | ||
| EmbA C76C | embA c228t | Beijing | |
| EmbA Q38Q | embA a114g | Subgroup of Beijing | |
| EmbC T1036T | embC c3108t | M. bovis | |
| EmbB N13S | M. bovis | ||
| EmbB V117V | embB c351t | AFRI-1 and M. bovis | |
| EmbB E378A | Ancestral MTBC lineages 1, 5, and 6 and M. bovis | ||
| EmbC T270I | Ancestral MTBC lineages 1, 5, and 6 and M. bovis | ||
| EmbB M423T | LAM4 from Portugal | ||
| EmbB P965P | embB g2895a | X | |
| EmbC R345R | embC c1035g | X2 | |
| Mutations potentially associated with ETB resistancea | EmbB M306V/Ib | ||
| EmbB G406C/S/A/Db | |||
| EmbB Q497Rb | |||
| EmbB F330I | |||
| EmbB Y334H | |||
| EmbB D354Ac | |||
| EmbB N399I | |||
| EmbB L402V | |||
| EmbB S426N | |||
| EmbB N296H | |||
| EmbB A19Dd | |||
| EmbA G5Vd | |||
| embCA -c8a/t | |||
| embCA -c12t | |||
| embCA -c16t | |||
| embCA with deletion of cg at positions −21 and −20 | |||
a
embCA is the embC-embA intergenic region.
b
Implication in ETB resistance proven by site-directed mutagenesis or allelic exchange.
c
Involvement in ETB resistance suggested by the in vitro-selected mutant.
d
Implication in ETB resistance remains to be experimentally verified.