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. 2015 Jul 16;59(8):4464–4470. doi: 10.1128/AAC.00501-15

TABLE 1.

Isolates and plasmids carrying blaCTX-M-15, blaCTX-M-64, blaCTX-M-123, or blaCTX-M-132

Isolatea blaCTX-M Date of isolation (mo/yr) Source Region/farmb E. coli MLST Plasmid markerc IncI2/orf477d IncI2/ISEcp1e Plasmid size (kb)f
IncI2
    JC2g 15 11/2006 Chicken SD17 224 pHNY2-1 P P 65,358
    AHC46h 64 6/2011 Chicken feces AH14 1011 pHNAH46-1 P P 62,194
    LDH19i 132 1/2013 Human urine GD- 4528 pHNLDH19 P P 62,194
    SG0514-2c 64 3/2012 Chicken heart GD07 117 P P P ∼62
    ACH5i 64 6/2011 Chicken feces AH03 162 P P P ∼62
    SG0532-2i 64 3/2012 Chicken heart GD07 3851 P P P ∼62
    GDC16i 64 8/2010 Chicken feces GD15 4474 P P P ∼62
    BSC1i 64 1/2010 Chicken feces GD13 4346 P P P ∼62
IncI1 ST108
    AHC4i 123 6/2011 Chicken feces AH04 746 pHNAH4-1 P NA 109,194
    AHC13h 123 6/2011 Chicken feces AH06 746 P P NA ∼113
    AHC14i 123 6/2011 Chicken feces AH06 746 P (no MRR1) P NA ∼113
    AHC2h 123 6/2011 Chicken feces AH03 746 P (no MRR1) P NA ∼113
    AHC54i 123 7/2011 Chicken feces AH02 155 P P NA ∼113
    NKSC61h 123 1/2011 Chicken liver GD07 155 P P NA ∼109
    AHC55h 123 7/2011 Chicken feces AH02 162 P P NA ∼113
    FKP358i 123 11/2010 Pig feces GD05 165 P P NA ∼113
    FKD567h 123 8/2012 Duck feces GD01 1437 P P NA ∼113
    NKSC1i 123 1/2011 Chicken liver GD07 2309 P P NA ∼105
Inc group not determined
    FKP614Fj 64 11/2011 Pig feces GD16 746 ND ND ND ND
    TP36g 123 6/2012 Chicken heart GD12 93 ND ND ND ND
    FKP587j 123 1/2011 Pig feces GD09 156 ND ND ND ND
    FKD453j 123 1/2011 Duck feces GD10 205 ND ND ND ND
    FKP97j 123 8/2010 Pig feces GD11 1437 ND ND ND ND
    FKP745j 123 8/2012 Pig liver GD08 1771 ND ND ND ND
a

Bold type indicates isolates from which plasmids were completely sequenced. Plasmid names are shown in the “Plasmid marker” column.

b

AH, Anhui Province; GD, Guangdong Province; SD, Shandong Province. Farms are numbered. LDH19 is from a human clinical isolate.

c

Transconjugants (Tc) or transformants (Tx) were screened for IncI2 or IncI1 (pHNAH4-1) markers, as appropriate, using primers in Table S1 in the supplemental material. P, positive; ND, not determined.

d

PCR with primers CHP1-F and Orf477-R (see Table S1), linking the IncI2 backbone fragment to orf477.

e

PCR with HP2-R and ISEcp1-F (see Table S1) linking the IncI2 backbone to ISEcp1. NA, not applicable.

f

Approximate plasmid sizes were estimated from S1 gels.

g

Gave Tx carrying blaCTX-M but more than one plasmid.

h

Gave Tx carrying a single plasmid with blaCTX-M.

i

Gave Tc carrying a single plasmid with blaCTX-M.

j

No Tc/Tx carrying the relevant blaCTX-M gene were obtained.