FIG 1.

Chemical-genetic profiling using HCGP assay identified key UPR regulators as required for carvacrol tolerance. (A) GO term enrichment of carvacrol sensitive mutants. The P value was calculated using the hypergeometric distribution. (B, C) Individual confirmations of the chemical-genetic screen by spot serial dilution assay. A total of three deletion mutants, the ire1, hac1, and aro2 mutants, were selected and spotted on YPD with DMSO (control), YPD containing 0.8 mM or 1 mM carvacrol (B) or 1.74 mM eugenol, 2.16 mM isopulegol, 2.22 mM l-(−)-carvone (vol/vol), or 2.22 mM d-(+)-carvone (C). Plates were incubated at 30°C for 2 days.