TABLE 1.

Chemical-genetic profiling of carvacrol^a

Gene name	Description of product	Z-score
IRE1	Serine-threonine kinase and endoribonuclease; mediates the unfolded protein response by regulating Hac1p synthesis through HAC1 mRNA splicing	3.03
HAC1	Transcription factor; regulates the unfolded protein response	2.83
TRP3	Indole-3-glycerol-phosphate synthase	1.92
ARO2	Chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate to form chorismate, which is a precursor to aromatic amino acids	1.87
ARO1	Pentafunctional arom protein; catalyzes steps 2 through 6 in the biosynthesis of chorismate	1.86
BCK1	MAPKKK acting in the protein kinase C signaling pathway	1.79
PHO84	Hig high-affinity inorganic phosphate transporter	1.63
YPT6	Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi complex	1.62
TRP2	Anthranilate synthase; catalyzes the initial step of tryptophan biosynthesis	1.56
TLG2	Syntaxin-like t-SNARE; mediates fusion of endosome-derived vesicles with the late Golgi complex	1.48
CWH41	ER type II integral membrane N-glycoprotein involved in assembly of cell wall β-1,6-glucan and asparagine-linked protein glycosylation	1.47
GTR2	Putative GTP binding protein; negatively regulates Ran/Tc4 GTPase cycle	1.36
SLT2	Serine/threonine MAP kinase involved in regulating maintenance of cell wall integrity, cell cycle progression, and nuclear mRNA retention	1.32
TRP4	Anthranilate phosphoribosyl transferase; transferase of the tryptophan biosynthetic pathway	1.30
MDM31	Mitochondrial protein that may have a role in phospholipid metabolism	1.22
SMI1	Protein involved in the regulation of cell wall synthesis	1.14
SWI6	Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; required for the unfolded protein response	1.13
COG6	Component of the conserved oligomeric Golgi complex; functions in protein trafficking to mediate fusion of transport vesicles to Golgi complex	1.12
YPS7	Putative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum	1.11
RPS8A	Protein component of the small (40S) ribosomal subunit	1.01
TRS85	Component of transport protein particle complex III; regulates endosome-Golgi complex traffic and required for membrane expansion	1.01
TRP1	Phosphoribosylanthranilate isomerase; catalyzes the third step in tryptophan biosynthesis	1.00

^aIdentification by HCGP assay of gene deletion mutants that confer sensitivity to carvacrol. Fitness defect scores were calculated based on bar code microarray hybridization, and the top 22 sensitive deletion strains sorted by Z-score are shown.