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. 2015 Jul 16;59(8):4669–4679. doi: 10.1128/AAC.04694-14

FIG 4.

FIG 4

T. cruzi sirtuins have opposite effects on parasite growth and differentiation. (A) Growth curves of wild-type (●), TcSir2rp1-ox (□), and TcSir2rp3-ox (■) epimastigotes. (B and C) Percentage of differentiation of epimastigotes into metacyclics by the wild-type (black bars), TcSir2rp3-ox (gray bars), and TcSir2rp1-ox (white bars) parasites in the absence (−) or presence (+) of 15 mM nicotinamide (Nic) (B) and 2 μM salermide (Sal) (C). All values are means ± SE (n = 3). The asterisks indicate statistically significant differences based on the Student t test (*, P < 0.01; ***, P < 0.003). (D) Immunoblotting of parasites after differentiation induction probed with anti-gp30. An epimastigote extract was used as the negative control (−), and anti-β-tubulin was used as the loading control.