TABLE 2.

Amino acid changes in NA of virus isolated from nasal samples after treatment with NA inhibitors

Treatment	Monkey	Nucleotide positiona	Inoculum nucleotideb	Sample allele nucleotidec	No. of readsd	Total no. of readse	% allelef	Amino acid positiong	AA in inoculumh	AA in samples
Saline solution	S1	96	C	T	19,642	49,403	39.76	32	N	Synonymousi
Saline solution	S2	814	A	T	32,571	84,385	38.60	272	I	F
Saline solution	S2	825	C	T	51,298	85,879	59.73	275	C	Synonymous
Saline solution	S2	1379	A	G	57,056	103,490	55.13	460	K	R
Saline solution	S3	510	G	A	28,761	127,797	22.51	170	V	Synonymous
Saline solution	S3	825	C	T	10,690	68,710	15.56	275	C	Synonymous
Saline solution	S3	1242	C	T	5,821	31,400	18.54	414	C	Synonymous
Saline solution	S3	1379	A	G	3,666	31,033	11.81	460	K	R
Oseltamivir	O2	656	T	C	102,728	103,880	98.89	219	I	T
Oseltamivir	O3	866	G	A	85,405	100,094	85.32	289	R	K
Peramivir	P1	866	G	A	79,827	88,187	90.52	289	R	K
Peramivir	P3	858	C	T	39,120	39,491	99.06	286	C	Synonymous

^aNucleotide position 1 is the first nucleotide A of the ATG translation start point for methionine.

^bViral genes were purified from the virus in the same stock lot as that used in inoculation of macaques on day 0.

^cNucleotides different from those in the inoculum virus. All nasal swab samples indicated in the table were collected 7 days after virus inoculation.

^dNumber of sequences with indicated nucleotide changes at each nucleotide position.

^eNumber of sequences analyzed at each nucleotide position.

^fPercentages of alleles different from the inoculum were calculated as percent allele = 100 × (number of alleles/total read number). Allele frequencies that were higher than 10% are shown in the table.

^gAmino acid (AA) positions were determined on the basis of an N9 neuraminidase sequence of Anhui/1.

^hC, cysteine; I, isoleucine; F, phenylalanine; K, lysine; N, asparagine; R, arginine; T, threonine; V, valine.

ⁱSynonymous, amino acids were not changed by nucleotide changes.