FIG 1.

Sequence variations and phylogenetic relationship between PBP2 variants of this study. (A) The altered positions in the amino sequences of wild-type PBP2 (M32091), H041 PBP2 (AB546858) (19), and the variants of this study are shown. The residue numbers of wild-type and mosaic variants were shifted by +1 to maintain alignment with the nonmosaic alleles, which contain an Asp insertion after position 345; thus A501P and A501V substitutions are shown at position 502. Dots indicate consensus with the wild-type sequence, while bold text indicates novel variants and substitutions. The last 12 alleles (I to XXII) are nonmosaic alleles. The variant named V MNP* was encoded by an allele containing multiple nucleotide polymorphisms (MNP) compared to variant V. (B) A dendrogram based on the amino acid sequences of the PBP2 variants of this study was created with ClustalX by the nearest neighbor-joining method. Highlighted branches contain either mosaic alleles or nonmosaic alleles with A501V substitutions.