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. 2015 Jul 17;5:12138. doi: 10.1038/srep12138

Figure 1. Rescue of F508del-CFTR by gene silencing.

Figure 1

The bar graph shows F508del-CFTR activity in CFBE41o- cells based on a YFP assay after transfection with siRNAs (10 nM final concentration) against indicated targets or with control, non-targeting (NT) siRNA (10 nM siRNA in all conditions). The activity measured upon treatments was normalized for the activity detected under control condition (NT-siRNA + DMSO). The assay was carried out 48 hours after transfection. Cells were incubated at 37 °C prior to analysis. Data are expressed as means ± SEM, n = 8. Statistical significance was tested by parametric ANOVA followed by the Dunnet multiple comparisons test (all groups against the control group). Symbols indicate statistical significance versus NT-siRNA: **P < 0.01, *P < 0.05; or versus NT-siRNA + VX-809: §§P < 0.01, §P < 0.05. A. Results of siRNA library screening. B. Additivity of target silencing with VX-809. Bar graph shows results of combined treatment with vehicle alone (DMSO, white bar) or VX-809 (1 μM; gray bars) of cells transfected with different siRNAs.