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. 2015 Jul 17;5:12276. doi: 10.1038/srep12276

Figure 4. MiR-135b-5p and miR-499a-3p promote endothelial cell proliferation and migration.

Figure 4

(A) Western blot analysis of MEF2C expression in HUVEC cells transfected with the indicated miRNAs mimics. The full-length gels are presented in Supplementary Fig. S7. (B) Compiled data from three independent experiments after transfection of miRNAs is shown. Columns, mean; Bars, ± SD; ***P < 0.001. (C) Western blot analysis of MEF2C expression in HUVEC cells transfected with the indicated miRNAs and pcDNA3.1A-MEF2C.The full-length gels are presented in Supplementary Fig. S7. (D) Compiled data from three independent experiments after transfection of miRNAs is shown. Columns, mean; Bars, ± SD; ***P < 0.001. (E) Photoimages of EdU incorporation assay of HUVECs that were treated with miRNAs or rescue plasmid. Results are representative data from three independent experiments. (F) Proliferation rates of HUVECs that were treated with miRNAs or rescue plasmid. Results are presented as means ± SD of three independent experiments. *P < 0.05; **P < 0.01. (G) Photoimages of scratch assay of HUVECs that were treated with miRNAs or rescue plasmid. Results are representative data from three independent experiments. (H) Migration rates of HUVECs that were treated with miRNAs or rescue plasmid. Results are presented as means ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001. (I) Photoimages of transwell assay of HUVECs that were treated with miRNAs or rescue plasmid. Results are representative data from three independent experiments. (J) Migration rates of HUVECs that were treated with miRNAs or rescue plasmid. Results are presented as means ± SD of three independent experiments. *P < 0.05; **P < 0.01.