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. 2015 Jul 17;5:10572. doi: 10.1038/srep10572

Figure 5. HBV mRNA with miR-122 response elements enhances viral expression and replication through regulation of cyclin G1.

Figure 5

(a–c) Huh7 cells were co-transfected with HBV replication plasmid (pHBV) and pcDNA3.1 carrying the wild type (WT) or mutant (Mut) HBV pgRNA fragment within miR-122 response elements or the empty pcDNA3.1 vector as a mock. The secretion of HBsAg and HBeAg was measured by ELISA (a). HBV DNA copy number in the supernatant was quantified by real-time PCR (b) HBV-DNA levels in cells were detected by Southern blotting (c) (d,e) Huh7 cells were co-transfected with HBV replication plasmid (pHBV) and pcDNA3.1 carrying the wild type (WT) or mutant (Mut) HBV pgRNA fragment or the empty pcDNA3.1 vector as a mock, along with cyclin G1 siRNA. HBsAg and HBV DNA levels were determined as in a and b. Data shown are the means ± SD for three independent experiments. * P < 0.05; ** P < 0.01.