Fig. 8. Effect of PLREE on mRNA expression of adipogenic, lipogenic, and lipolytic genes during the differentiation of 3T3-L1 preadipocytes into adipocytes. (A) 3T3-L1 preadipocytes were treated with PLREE for 8 days, and mRNA levels of adipogenic, lipogenic, and lipolytic genes in 3T3-L1 cells (day 8) were analyzed by semi-quantitative RT-PCR. Expression was normalized to GAPDH levels. (B) Quantification of mRNA levels of adipogenic, lipogenic, and lipolytic genes. Values represent the mean ± SD of three independent measurements. *p < 0.05, **p < 0.01 compared to differentiated control cells. N: undifferentiated normal cells, C: differentiated control cells.