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. 2015 Jun;31(2):191–201. doi: 10.5487/TR.2015.31.2.191

Table 1. Primer sequences for target genes used in 3T3-L1 cells for PCR.

Genes Primers Expected size (bp)1)

SREBP-1c Forward (5'→3') GCG CTA CCG GTC TTC TAT CA 234
Reverse (5'→3') TGC TGC CAA AAG ACA AGG G
C/EBPβ Forward (5'→3') GAC TAC GCA ACA CAC GTG TAA CT 488
Reverse (5' →3') CAA AAC CAA AAA CAT CAA CAA CCC
PPARγ Forward (5'→3') GGT GAA ACT CTG GGA GAT TC 268
Reverse (5' →3') CAA CCA TTG GGT CAG CTC TC
C/EBPα Forward (5'→3') TTA CAA CAG GCC AGG TTT CC 232
Reverse (5'→3') CTC TGG GAT GGA TCG ATT GT
ACC Forward (5'→3') TGA CCG TGG GCA CAA AGT T 351
Reverse (5'→3') AGG AGG AAC CGC ATT TAT CGA
FAS Forward (5'→3') GGT TCG GAA TGC TAT CCA GG 301
Reverse (5'→3') CTG CGG AAA CTT CAG GAA AT
Leptin Forward (5'→3') CCA AAA CCC TCA TCA AGA CC 352
Reverse (5'→3') CTC AAA GCC ACC ACC TCT GT
ATGL Forward (5'→3') TTC ACC ATC CGC TTG TTG 155
Reverse (5'→3') AGT TCC ACC TGC TCA GAC
HSL Forward (5'→3') GTC GCC TTT CTC CTG ATG AC 410
Reverse (5'→3') CTT GCT GCT TCT CTT GGC TC
GAPDH Forward (5'→3') GTA TGA CTC CAC TCA CGG CAA A 248
Reverse (5'→3') GGT CTC GCT CCT GGA AGA TG

1)bp: basepair.