Table 1. Primer sequences for target genes used in 3T3-L1 cells for PCR.
Genes | Primers | Expected size (bp)1) |
---|---|---|
| ||
SREBP-1c | Forward (5'→3') GCG CTA CCG GTC TTC TAT CA | 234 |
Reverse (5'→3') TGC TGC CAA AAG ACA AGG G | ||
C/EBPβ | Forward (5'→3') GAC TAC GCA ACA CAC GTG TAA CT | 488 |
Reverse (5' →3') CAA AAC CAA AAA CAT CAA CAA CCC | ||
PPARγ | Forward (5'→3') GGT GAA ACT CTG GGA GAT TC | 268 |
Reverse (5' →3') CAA CCA TTG GGT CAG CTC TC | ||
C/EBPα | Forward (5'→3') TTA CAA CAG GCC AGG TTT CC | 232 |
Reverse (5'→3') CTC TGG GAT GGA TCG ATT GT | ||
ACC | Forward (5'→3') TGA CCG TGG GCA CAA AGT T | 351 |
Reverse (5'→3') AGG AGG AAC CGC ATT TAT CGA | ||
FAS | Forward (5'→3') GGT TCG GAA TGC TAT CCA GG | 301 |
Reverse (5'→3') CTG CGG AAA CTT CAG GAA AT | ||
Leptin | Forward (5'→3') CCA AAA CCC TCA TCA AGA CC | 352 |
Reverse (5'→3') CTC AAA GCC ACC ACC TCT GT | ||
ATGL | Forward (5'→3') TTC ACC ATC CGC TTG TTG | 155 |
Reverse (5'→3') AGT TCC ACC TGC TCA GAC | ||
HSL | Forward (5'→3') GTC GCC TTT CTC CTG ATG AC | 410 |
Reverse (5'→3') CTT GCT GCT TCT CTT GGC TC | ||
GAPDH | Forward (5'→3') GTA TGA CTC CAC TCA CGG CAA A | 248 |
Reverse (5'→3') GGT CTC GCT CCT GGA AGA TG |
1)bp: basepair.