Table 2. PCR conditions for target genes in 3T3-L1 cells.

Genes	Items	Temperature	Time	Cycles
SREBP-1c	Predenaturation	95℃	10 min	1
	Denaturation	95℃	1 min	30
	Annealing	54℃	1 min
	Extension	72℃	1 min
	Post extension	72℃	10 min	1
C/EBPβ	Predenaturation	95℃	10 min	1
	Denaturation	94℃	30 sec	30
	Annealing	53℃	30 sec
	Extension	72℃	30 sec
	Post extension	72℃	7 min	1
PPARγ	Predenaturation	95℃	10 min	1
	Denaturation	94℃	30 sec	30
	Annealing	53℃	30 sec
	Extension	72℃	30 sec
	Post extension	72℃	7 min	1
C/EBPα	Predenaturation	94℃	10 min	1
	Denaturation	94℃	30 sec	30
	Annealing	62℃	30 sec
	Extension	72℃	30 sec
	Post extension	72℃	7 min	1
ACC FAS	Predenaturation	94℃	5 min	1
	Denaturation	94℃	30 sec	30
	Annealing	56℃	1 min
	Extension	72℃	1 min
	Post extension	72℃	5 min	1
Leptin	Predenaturation	94℃	1 min	1
	Denaturation	94℃	1 min	33
	Annealing	57℃	1 min
	Extension	72℃	2 min
	Post extension	72℃	7 min	1
ATGL HSL	Predenaturation	94℃	5 min	1
	Denaturation	94℃	45 sec	29
	Annealing	53℃	45 sec
	Extension	72℃	90 sec
	Post extension	72℃	5 min	1