FIGURE 4.

c-Abl inhibits degradation of HIPK2. A, HEK293 cells were transfected with pEFIRES FLAG-HIPK2 and c-Abl Δ1–81, as indicated. Cycloheximide (CHX) (500 μm) was added 24 h after transfection, and cells were harvested at the times indicated. Cells were analyzed by SDS-PAGE and immunoblotting. Quantification of the HIPK2 signal relative to tubulin is plotted in the graph below. B, HEK293T cells were transfected with pEFIRES FLAG-HIPK2 WT and Y360F (full-length clones), HA-Siah-1, and c-Abl Δ1–81 WT or kinase-mutant (Km), as indicated. Cells were harvested 25 h after transfection and were analyzed by SDS-PAGE and immunoblotting with the indicated antibodies.