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Blood-fed mosquito midguts bound significantly (p < 0.001) more High Five cell-expressed recombinant FREP1 compared with unfed (naive) mosquito midguts. Purified recombinant FREP1 or irrelevant protein (BSA) was incubated with midguts and the midgut-bound FREP1 was quantified by ELISA. Data (mean ± S.D.) are from three independent experiments.
