TABLE 1.

PCR primers

The italic and underlined sequences denote restriction recognition sites. The underlined sequences are T7 promoter. The bold sequence is Kozak consensus sequences. The primers were synthesized through Integrated DNA Technologies Inc.

Purpose	Primer name	Primer sequence
In vitro synthesis of FREP1 dsRNA	Forward	5′-TAATACGACTCACTATAGGAGCTCGAGGTGAAGCAGAG-3′
	Reverse	5′-TAATACGACTCACTATAGGTTCTCCAGCCGGTTGTGT-3′
Verify FREP1 mRNA expression	Forward	5′-ACAGGGCAAGTTCGAGAAGA-3′
	Reverse	5′-AAGTCAACCGTACCGTCCTG-3′
AgS7 gene	Forward	5′-GGCGATCATCATCTACGTGC-3′
	Reverse	5′-GTAGCTGCTGCAAACTTCGG-3′
In vitro synthesis of GFP dsRNA	Forward	5′-TAATACGACTCACTATAGGCAAGTTTGAAGGTGATACCC-3′
	Reverse	5′-TAATACGACTCACTATAGGCTTTTCGTTGGGATCTTTCG-3′
Clone FREP1 into pQE30	Forward	5′-ACCCGGGCACTGCCCTGAACGGTGCAG-3′
	Reverse	5′-GGCAAGCTTCGCGAACGTCGGCACAGTC-3′
Clone FREP1 into pIB/V5-His	Forward	5′-TCAAAGCTTCACCATGGTGAATTCATTCGTGTCG-3′
	Reverse	5′-ACTCTAGATTACGCGAACGTCGGCACAGTCGTG-3′
	Reverse with His tag	ACTCTAGAGCGAACGTCGGCACAGTCGTG